Margot du Jonchay first trained in contemporary dance at the Paris Conservatoire, then in theater at the Conservatoire du 19e arrondissement. A graduate of the École Nationale Supérieure d'Arts de Paris-Cergy and with a Master's degree in directing from the Académie Royale des Beaux-Arts in Brussels, she works mainly in the performing arts and cinema as a director, dancer and actress. In 2016, she played Caroline in Cigarettes et chocolat chaud by Sophie Reine (Mandarin Cinéma). More recently, she played in Open Heart by Kohki Maeda (La Fémis) and Le perdant radical by Ugo Simon (Don Quichotte Film).

At the same time, she is developing a documentary theater project with Odran Guillemard, based on the parliamentary debates on assisted dying in France.